Tips & Recommendations for PCR Beginners
We asked the science community on Twitter to share their favorite tips for PCR beginners:
“When using strips, BE SURE to label one end of the strip to know your reaction order. It’s easy to mix up which end you started on.”@IHStreet
“Patience, patience, patience…And yeah, always controls. No exceptions.”@GoldenMole
“Don’t forget to write down which well has your negative control. Also, PCR is witchcraft.”@SFriedScientist
“Don’t mix flat and dome caps in the same cycle!”@IHStreet
“Always run a control, run your samples in duplicate, use a gradient PCR machine, and take detailed notes.”@HeidiKayDeidi